MGPC's aim is to offer support to the Biochemistry and Molecular Medicine department’s faculty, students, and other researchers in the School of Medicine and Health Sciences. Below is a list of the analysis available through MGPC for investigators
Data management or storage options
Options available for data storage include MGPC servers, Colonial One, or AWS. For more information email: hadley_king@gwu.edu
Analytical pipelines
- Absolute and comparative abundance of expression (FPKM)
- Next generation whole transcriptome sequencing: in silico and in vitro collaborative venues
- Splicing: novel isoforms, absolute and comparative abundance of novel and known isoforms
- Variations: novel and known variants
Post-analytical pipelines
- Integration of variation, expression, and splicing in search for novel regulatory networks
- Comparative expression and variation analysis with population and cancer-centered public datasets
- Extraction of cancer related features: loss-of-heterozygosity, allele-preferential expression, RNA editing, disease-driving somatic changes
- Noncoding transcriptome
- Selected gene or pathway focused analyses: alignment of within user’s dataset info with the public sources on specific genes and pathways of interest
- Custom user-generated analyses and queries
- Advise for wet-lab validation
Epigenomic pipelines
- Mapping of ChIP-seq reads to reference genome - coding and non-coding regions
- Peak calling
- Linking with histone marks
- Analysis of peak co-ordinates and associated gene-annotation
- MOTIF analysis
Proteome-wide analysis of effects of non-synonymous variation
- Analysis of functional impact of variation on active site, binding sites, pathways and domains
- Analysis of effects of variation on Post-translational modifications
Metagenomic analysis
- Analysis of short-reads from metagenomes to determine taxonomic distribution
Phylogentic analysis using SNP data
- Create phylogenetic trees from SNP data to classify samples
For analysis of RNA-related or DNA mutation calling email: ahorvath@gwu.edu
For analysis of metagenomic data, HIV and HCV quasispecies analysis, or analysis using the HIVE platform email: hadley_king@gwu.edu